Physical properties of chondroitin sulphate/dermatan sulphate proteoglycans from bovine aorta
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چکیده
منابع مشابه
Bovine aortic chondroitin sulphate- and dermatan sulphate-containing proteoglycans. Isolation, fractionation and chemical characterization.
1. Guanidinium chloride (4M) in the presence of proteinase inhibitors extracted 90% of bovine aorta galactosaminoglycans as proteoglycans that were subsequently purified by ion-exchange and gel chromatography. 2. Fractionation of the calcium salts of the purified proteoglycans with increasing concentration of ethanol yielded fractions PG-25 (28%), PG-35 (45%) and PG-50 (37%). 3. Fraction PG-50 ...
متن کاملIsolation and characterization of dermatan sulphate proteoglycans from bovine sclera.
1. Proteoglycans were extracted from sclera with 4 M-guanidine hydrochloride in the presence of proteinase inhibitors and purified by ion-exchange chromatography and density-gradient centrifugation. 2. The entire proteoglycan pool was characterized by compositional analyses and by specific chemical (periodate oxidation) and enzymic (chondroitinases) degradations. The glycan moieties of the mole...
متن کاملCharacterization of the keratan sulphate proteoglycans from bovine corneal stroma.
The keratan sulphate proteoglycans that can be prepared from bovine corneal stroma [Axelsson & Heinegård (1975) Biochem. J. 145, 491-500] were characterized by gel chromatography, gel electrophoresis and analytical ultracentrifugation in associative (0.6 M-NaCl) and dissociative (6M-guanidinum chloride) solvents. The proteoglycans aggreagated at low salt concentrations and pH. The weight-averag...
متن کاملIsolation and characterization of proteoglycans from bovine aorta.
Proteoglyeans from bovine aorta were extracted by 0.15 M NaCl and by digestion with collagenase and with elastase. The nonspecific protease activity of elastase was inhibited by soybean trypsin inhibitor. Glycosaminoglycans (GAG) of the proteoglycans were isolated, characterized, and quantitated. About 50% of the tissue chondroitin 4and B-sulfates, 75% of hyaluronic acid, and almost all chondro...
متن کاملStructure and properties of dermatan sulphate proteoglycans.
Introduction Fibrous connective tissues like sclera, skin, tendon and cornea have a high content of collagen but only small amounts of proteoglycans and structural glycoproteins. The specific blend of collagen and proteoglycan types determines the properties of the tissue. The proteoglycans are characterized by having a central protein core substituted by one or several glycosaminoglycan chains...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1986
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2400575